{"id":3750,"date":"2024-01-08T09:28:19","date_gmt":"2024-01-08T09:28:19","guid":{"rendered":"https:\/\/agrinet.ir\/eng\/?p=3750"},"modified":"2024-01-08T10:08:59","modified_gmt":"2024-01-08T10:08:59","slug":"blueberry-tissue-culture","status":"publish","type":"post","link":"https:\/\/agrinet.ir\/eng\/blueberry-tissue-culture\/","title":{"rendered":"Blueberry tissue culture"},"content":{"rendered":"

Blueberry propagation protocol by tissue culture method<\/span><\/h2>\n

Blueberry (Vaccinieae, Vaccinioideae, Ericaceae<\/em>) is a deciduous or evergreen shrub. Blueberry fruit contains anthocyanin, unsaturated fatty acids, calcium, potassium, zinc, iron, and other elements. The blueberry tissue culture method helps us to proliferate this valuable plant.<\/span><\/p>\n

Blueberries are recognized as one of the top five health foods by the International Food and Agriculture Organization. Also, the fruit of this plant is known as the king of fruits due to the presence of antioxidants and its high properties. This has caused the fruits of the Berry family to become one of the world’s most promising fruit tree species for human nutrition and health in the future.<\/span><\/p>\n

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\"Blueberry<\/span><\/p>\n

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In recent years, with the increasing understanding of the impact of blueberry fruit on human health, the demand for consumption of the fruit of this plant in the world markets has increased steadily. This sharp increase in demand has caused the rapid expansion of the blueberry production industry, especially in the seedling and planting stage. Therefore, there is an urgent need for the quick production of blueberry seedlings.<\/span><\/p>\n

As market demand for blueberries continues to improve, the scale and area of planting continue to expand, and yields have increased significantly. The multiplication factor of this plant through grafting is slow-going. The grafting is challenging for plants’ proliferation and quality supply to the market.<\/span><\/p>\n

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\"Blueberry<\/span><\/p>\n

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Plant tissue culture is a technology in which the tissue, organ, and cell isolated from the plant are cultured aseptically in an environment containing nutrients and plant hormones, and the whole plant is regenerated.<\/span><\/p>\n

Plant tissue culture has significant advantages in growing seedlings, such as high propagation speed, seedling health, high quality and efficiency, and no time limit. The production of blueberry seedlings by tissue culture method is rapidly expanding and becoming popular in the world markets.<\/span><\/p>\n

Materials and methods<\/span><\/h3>\n

Selection and surface sterilization of explants:<\/span><\/p>\n

The selected explants are best obtained from the buds of young leaves (without petioles) and shoots (without leaves) of completely healthy two-year-old blueberries kept in the Screen House. Different methods are used for sterilization, and below mentioned the two most used methods:<\/span><\/p>\n

The first method of sterilizing blueberry seedlings<\/span><\/h4>\n

The selected explants were washed under running water for 6 minutes and then sterilized five times with ddH2O. In the next step, explants are sterilized with 75% alcohol for 20 seconds and then with 2% (v\/v) NaClO for 5 to 8 minutes, respectively. Finally, sterilized explants should be washed with distilled water.<\/span><\/p>\n

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\"Blueberry<\/span><\/p>\n

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The second method of sterilizing blueberry seedlings<\/span><\/h4>\n